Based on the patient derived-xenografts (PDX) animal disease model of cancer , We established the PDX model using human primary tumor cells as explants in BALB/c nude mice to construct in situ and ectopic tumor models.

It maintains the heterogeneity of the tumor , and retains the short modeling time of the common CDX model (cell strain), achieving the overall traceability from human primary cells to the model. Meanwhile, our PDX model use human primary tumor cells as explants at low passage numbers(average of six passes), which is believed to conserve original tumor characteristics such as heterogeneous histology, clinical biomolecular signature, malignant phenotypes and genotypes, tumor architecture, and tumor vasculature.