平台简介 本科学实验服务平台是莱艾特科技联合中国农业大学科研团队,携手美国芝加哥大学、加拿大McMaster大学、中科院、中国检科院、北京市营养源研究所等国内外一流大学、科研机构和企业资深专家,搭建的技术服务平台。运用国内外先进技术与设备、洁净动物房和良好的实验室,为生命科学实验提供食品、药品安全评估、营养保健品功能评价、动物疫病诊断等技术服务。 | |||
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| 整体实验外包 | | 动物实验操作 | | 小动物活体成像 | | 动物行为学 | |
| 深度分析数据及论文指导 | | 动物分组 | | 活体成像仪使用 | | 水迷宫 | |
| 课题开展与管理 | | 体重监测 | | 活体成像 | | 十字迷宫 | |
| 实验方案设计指导 | | 肿块监测 | | 动物模型 | | 八壁迷宫 | |
| 文献分析与选题指导 | | 实验给药操作 | | 肠炎模型 | | Y迷宫 | |
| 实验动物寄养 | | 动物麻醉操作 | | 肥胖与糖尿病模型 | | T迷宫 | |
| SPF级大、小鼠饲养 | | 动物解剖及组织提取 | | 非酒精性脂肪肝 | | 矿场实验 | |
| SPF级大、小鼠高脂饲养 | | 采血及分离血清 | | | | 基因型鉴定 | |
| 普通级兔、犬、猴、猫、猪、牛、羊饲养 | | 尸体处理 | | | | | |
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| 制片前处理 | | 染色 | | 尼氏染色 | | 免疫技术 | |
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骨组织脱钙 | | HE染色 | |
肥大细胞染色 | | 免疫组化 | |
| 组织脱水 | | 番红固绿(植物/软骨)染色 | | 苯胺蓝染色 | | 免疫荧光(单染) | |
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石蜡包埋 | | Masson染色 | | LFB髓鞘染色 | | 免疫荧光(双染) | |
| OCT包埋 | | 天狼猩红染色 | | 普鲁士蓝染色 | | 免疫组化芯片 | |
| 石蜡白片 | | PAS糖原染色 | | VG/EVG染色 | | | |
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组织冰冻切片 | |
阿利新蓝染色 | | 维多利亚染色 | | 病理阅片及报告 | |
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组织冰冻切片 | | AB-PAS染色 | | von kossa染色 | | 拍照/扫描 | |
| 硬组织切片 | | 油红O(切片)染色 | | 茜红素染色 | | 阅片/读片 | |
| 摊片烤片 | | 瑞氏吉姆萨染色 | | 抗酸染色 | | 病理诊断 | |
| 切片封片 | | 甲苯胺蓝染色 | | 富尔根染色 | | 病理报告 | |
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| 生化分析检测 | | 血常规检测 | | 氨基酸 | 脂肪酸分析 | | 药残 | 微生物检测 | |
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前处理-组织匀浆 | |
白细胞 | 红细胞 | 血红蛋白 | | 游离脂肪酸 | | 盐酸克伦特罗 | 四环素 | |
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丙氨酸氨 | 天门冬氨酸基转移酶 | |
单核细胞数 | 单核细胞比率 | | 短链脂肪酸 | |
莱克多巴胺 | 沙丁胺醇 | 己烯雌酚 | |
| γ-谷氨酰基转移酶 | 脂肪酶 | |
嗜酸细胞数 | 嗜酸细胞比率 | | 18种不饱和脂肪酸 | |
黄曲霉素 | 伏马毒素 | 氰化物 | |
| 总胆红素 | 直接胆红素 | |
淋巴细胞数 | 淋巴细胞比率 | |
氨基酸分析 | |
沙门氏菌 | 细菌总数 | 大肠菌群 | |
| 尿素 | 肌酐 | 总蛋白 | 白蛋白 | |
中性粒细胞数 | 中性粒细胞比率 | |
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甘油三酯 | 总胆固醇 | |
红细胞压积 | 淋巴细胞数 | | 细胞流式 | | 其它检测实验 | |
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高密度 | 低密度脂蛋白胆固醇 | | 血小板 | 血小板平均体积 | | 细胞培养 | 原代细胞培养 | | 微量元素含量检测 | |
| 葡萄糖 | 尿酸 | 乳酸脱氢酶 | | 血小板体积分布宽度标准差 | | 流式细胞培养 | 流式细胞检测 | | 饲料概略养分分析 | |
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| 实时荧光定量PCR | | Western Blot检测服务 | | 质粒扩增与提取 | | |
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定量PCR标准曲线构建 | qPCR相对定量 | |
细胞 | 组织蛋白提取 | | 凝胶阻滞迁移电泳(EMSA) | |
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定量PCR标准品构建 | 基因组DNA抽提 | |
Western Blot | | 染色质免疫沉淀(ChIP) | |
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Taqman探针设计与合成 | RNA提取+反转录 | |
考马斯亮蓝染色 | | 免疫共沉淀(Co-Ip) | |
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qPCR引物设计合成 | RNA提取+反转录 | |
明胶酶谱 | | | |
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科学实验一站式服务平台
Have You Heard? 2020 update to AVMA Guidelines
l Gas flow rate calculated automatically for your cage size
l Key lockout prevents unauthorized tampering with controls
l Consistent gas delivery
l Run timer for ease of documentation
l Works with common rodent chambers or cages
l Lids available in several sizes
l Includes quick release fitting and hoses to connect to chamber or cage lid
l Adapters available to fit your current cage systems. Please contact us.
l CE Mark
l Made in the USA
Feature | Quietek 1 | Quietek XL |
One-touch operation. Just press “Start” Button. | √ | √ |
Key lockout to prevent tampering with controls | √ | √ |
Adjustable flow rate from 10 – 30% chamber volume/min. Custom flow rates available | √ | √ |
For the euthanasia of mice, rats, hamsters, guinea pigs, gerbils, degus and cotton rats | √ | √ |
Cage or chamber size to be controlled | 1 cage, 3 – 30 L | 1 cage, 30 – 200 L |
Dual settings for two different cage or chamber volumes | √ | √ |
Available with RapidFill™, for maximum flow rate after anesthetization, for the shortest, humane and compliant cycle times | √ | √ |
Has CE Mark | √ | √ |
Quietek models are designed for different sizes and numbers of cages. The Quietek 1 is suitable for cages between 3 and 30 liters in volume, and can be used with one chamber at a time. For larger chamber sizes (30 to 200 liters), we offer the Quietek XL.
Accessories
Accessories include spare keys and replacement tubing in the color of your choice.
If you need additional fittings, other tubing, or anything else, please contact us.
1.Cage Lid
Cage Lid – 13x 9 in. (33 x 22 cm.)
Cage Lid – 16x11 in. (40 x 28 cm.)
Cage Lid – 18x14 in. (45 x 35 cm.)
Cage Lid – 21x11 in. (53 x 28 cm.)
Cage Lid – 24x17 in. (60 x 43 cm.)
2.Carbon Dioxide Regulator
High flow regulator allows inlet pressure control of CO2 for any of the Quietek™ models. CGA320 inlet adapts to a standard USA carbon dioxide cylinder and 1/4″ barb outlet is ideal for connection to the Quietek. Automatic blow-off releases line pressure if in excess of 50 PSI to prevent damage to the Quietek.
3.Quietek Inlet Tubing
This 10 foot coil of polyester braided PVC tubing is ideal for connecting a Quietek to a regulator or centrally plumbed CO2 system. 1/4 inch inner diameter (ID) tubing is rated for 350 psi.
4.Quietek™ Tubing
6 feet of tubing to connect the Quietek to a cage lid. Comes with a quick connect fitting. Comes in a Blue, Green, Red, Yellow or Clear hose.
5.Quietek™ Keys
Quietek replacement keys. Set of 2.
International Guidelines
Now you can ensure that your laboratory rodents are humanely euthanized without disrupting your laboratory workflow. Introducing the Quietek™, the affordable, fully-automated device specifically designed by us to ensure compliance with strict international guidelines.
United States: “An optimal flow rate for CO2 euthanasia systems should displace 10% to 30% of the chamber or cage volume/min. Prefilled chambers are unacceptable.” 2013 AVMA Guidelines (pg. 49)
Canada: “…a gradual-fill rate of less than 30% and greater than 20% of the chamber volume per minute should be used.” CCAC Guidelines on: Euthanasia of Animals Used in Science (pg. 19)
United Kingdom: “Exposure to carbon dioxide gas in a rising concentration: appropriate for rodents and rabbits and birds up to 1.5kg…a controllable, metered source of carbon dioxide gas should be used.” The Humane Killing of Animals under Schedule 1 to the Animals (Scientific Procedures) Act 1986 (pg. 6)
European Union: “Only to be used in gradual fill.” Official Journal of the European Union (pg. 72, 73)
Australia: “a gradual fill of the chamber with 100% CO2 at a rate to fill 20% of the chamber volume per minute was agreed as good practice” Guidelines to Promote the Well-being of Animals Used for Scientific Purposes (pg. H3)
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