腹腔注射,维持时长10-40min,非管制药品无需公安报备,使用简单方便。
注意:本产品仅能用于实验小鼠,任何其他动物(兔、狗、猫、人等)均无任何效果,即使加大剂量也无效!
【适用范围】
本产品仅能用于实验大、小鼠,任何其他动物(兔、狗、猫、人等)均无任何效果,即使加大剂量也无效!
【产品概述】
经典的1.25%阿佛丁(2,2,2-三溴乙醇),腹腔注射,维持时长10-40min,非管制药品无需公安报备,使用简单方便。
【规格】
30mL
【贮藏】
4℃避光保存,有效期为两年。
【用法及剂量】
●本产品属于无菌即用型试剂,腹腔注射即可,使用方法简便。使用成本优于小动物麻醉机,麻醉效果好于水合氯醛,手术过程大、小鼠无反射,安全剂量范围大,不易死亡。
●使用剂量:小鼠的注射剂量为0.2mL/10g,大鼠的注射剂量为10mL/kg,腹腔注射。预实验需要尽早做,以确定剂量。
●麻醉时间:小鼠5分钟内可完全麻醉,维持麻醉大约10-40分钟,如手术时间需要延长,可在小鼠苏醒前腹腔补注射0.3mL-0.5mL麻醉剂。
●腹腔给药:以小鼠为例,大鼠的给药方法类似。左手抓住小鼠,使腹部向上,右手将注射针头于左(或右)下腹部30度角刺入皮下使针头向前推2-3mm左右,再以45度角穿过腹肌,固定针头,缓缓注入药液,为避免伤及内脏,可使小鼠处于头低位,使内脏移向上腹。
●不良反应:阿佛丁对小鼠中枢神经系统有抑制作用,给药量一旦过大可能会导致小鼠较长时间恢复甚至引起死亡。以C57小鼠为例,当麻醉剂的量超过标准给药时小鼠会逐步出现不良反应甚至死亡。当给药量是1.5倍标准给药量时小鼠行动迟缓,恢复常态时间较标准给药量长,有的小鼠超12小时才能爬起来且摇晃行走。当给药量是2倍标准给药量时部分小鼠反应比较激烈,给药后挣扎不到20秒便倒地,数小时后可见部分小鼠死亡。
参考文献 | |
三溴乙醇对小鼠麻醉效果的研究(点击标题查看原文) | |
摘要:为研究三溴乙醇对小鼠的麻醉效果,使用5组不同剂量三溴乙醇(200、350、500、800和1000mg/kg)分别腹腔注射小鼠,进行小鼠麻醉。结果表明, 200mg/kg三溴乙醇腹腔注射小鼠,麻醉效果很差,小鼠未达到麻醉状态而持续动弹,350~800mg/kg三溴乙醇腹腔注射小鼠能达到很好的麻醉效果。 关键词:三溴乙醇;麻醉;小鼠;效果 结果与分析:不同剂量三溴乙醇麻醉效果见表1 。除200mg/kg三溴乙醇麻醉效果很差,小鼠未达到麻醉效果而持续动弹外, 其余4组麻醉起效时间大约在3min 左右;350mg/kg 三溴乙醇腹腔注射小鼠,麻醉持续时间(58±7 )min ,镇痛效果、肌肉松弛度较好;500mg/kg 三溴乙醇腹腔注射小鼠,麻醉持续时间( 187±23 )min ,镇痛效果、肌肉松弛度都很好,麻醉持续时间较长; 800mg/kg 三溴乙醇腹腔注射小鼠,麻醉持续时间(507±46 )min;以1000 mg/kg三溴乙醇腹腔注射小鼠,(6±2 )min死亡, 而350~800mg/kg三溴乙醇剂量麻醉零死亡。 | |
Abstract:There is an art and science to performing mouse anesthesia, which is a significant component to animal research.Frequently, anesthesia is one vital step of many over the course of a research project spanning weeks, months, or beyond. It is critical to perform anesthesia according to the approved research protocol using appropriately handled and administered pharmaceutical-grade compounds whenever possible. Sufficient documentation of the anesthetic event and procedure should also be performed to meet the legal, ethical, and research reproducibility obligations. However, this regulatory and documentation process may lead to the use of a few possibly oversimplified anesthetic protocols used for mouse procedures and anesthesia. Although a frequently used anesthetic protocol may work perfectly for each mouse anesthetized, sometimes unexpected complications will arise, and quick adjustments to the anesthetic depth and support provided will be required. As an old saying goes, anesthesia is 99% boredom and 1% sheer terror. The purpose of this review article is to discuss the science of mouse anesthesia together with the art of applying these anesthetic techniques to provide readers with the knowledge needed for successful anesthetic procedures. The authors include experiences in mouse inhalant and injectable anesthesia, peri-anesthetic monitoring, specific procedures, and treating common complications. This article utilizes key points for easy access of important messages and authors’ recommendation based on the authors’ clinical experiences. Key words: anesthesia, animal research, animal welfare, Mus musculus, refinement | |
Abstract:To evaluate the phenotypic variation within a commercial outbred mouse stock, we examined sleep-time (or duration of loss of righting reflex) of outbred ICA mice after i.p. injectlon of ethanol (4.0 g/kg of body weight), urethane (1.3 g), tribromoethanol (250 mg), and pentobarbital (60 mg), and after i.v. injection of propofol (30 mg). We observed high-grade individual differences in sleep-time that ranged from 0 to 179 min,83.1 土 4.3 (mean and SEM of 100 mice) for ethanol: 0 to 169 min, 64.5 + 3.1 for pentobarbital; 0 to 160 min, 36.6 3.6 for urethane; 0 to 120 min, 21.5 2.2 for tribromoethanol: and 3 to 20.5 min, 7.1 0.3 for propofol. This extensive phenotypic variance within the outbred stock was as great as the variation reported among inbred strains or selected lines, and the varied susceptibility within the colony was inherited by Jcl:ICR-derived inbred strains IAl, ICT, IPl, and IQ1. The range of sleep-time variance for ethanol, pentobarbital, urethane, tribromoethanol, and propofol within four-way cross hybrid Jcl:MCH(ICR) mice was 86.6%, 63.3%,124%, 61.0%, and 53.1% that of outbred Jcl:ICR mice, respectively. The present study indicates that phenotypic variance within an outbred Jcl:ICR stock was at high risk for susceptibility to the drugs that depress the central nervous system and that Jcl:/CA-derived inbreds may be an excellent source of animal models for studying the anesthesia gene. Key words: hypnotic sleep, ICR-derived inbred strain, outbred ICR mice | |
The effects of anesthesia on measures of nerve conduction velocity in male C57Bl6/J mice(点击标题查看原文) | |
Abstract:Animal models,particularly mice,are used extensively to investigate neurological diseases. Basic research regarding animal models of human neurological disease requires that the animals exhibit hall mark characteristics of the disease. These include disease specific anatomical, metabolic and behavioral changes.Nerve conduction velocity (NCV) is the predominant method used to assess peripheral nerve health. Normative data adjusted for age, gender and height is available for human patients; however, these data arenot available for most rodents including mice. NCV may be affected by animal age and size, body temperature, stimulus strength and anesthesia. While the effects of temperature, age and size are documented,the direct and indirect effects of anesthesia on NCV are not well reported. Our laboratory is primarily concernedwithanimalmodelsofdiabeticneuropathy(DN)andusesNCVtoconfirmthepresenceofneu-ropathy. To ensure that subtle changes in NCV are reliably assayed and not directly or indirectly affected by anesthesia, we compared the effects of 4 commonly used anesthetics, isoflurane, ketamine/xylazine,sodiumpentobarbitaland2-2-2tribromoethanolonNCVinacommonlyusedrodentmodel,theC57Bl6/J mouse. Our results indicate that of the anesthetics tested, isoflurane has minimal impact on NCV and is the safest, most effective method of anesthesia. Our data strongly suggest that isoflurane should become. the anesthetic of choice when performing NCV on murine models of neurological disease. Key words: Sciatic nerve,Sural nerve,Isoflurane,2-2-2 Tribromoethanol,Surface temperature. | |
Anesthetic Effects of Tribromoethanol on Mice(点击标题查看原文) | |
Abstract:To study anesthetic effects of tribromoethanol on mice , the tribromoethanol were processed intraperitoncal injection with five groups of different dose gradients ( 200 、 350 、 500 、 800 and 1 000 mg/kg ) respectively , and then the anesthesia onset time and duration time were compared. Results showed that the tribromoethanon at dosage of 200 mg/kg showed the worst anesthesia effect , the mice were unanesthetized and kept moving ; intraperitoncal injection of tribromoethanol at dosage of 350~800 mg/kg had moderate anesthesia duration and adequate anesthesia depth , which were suitable anesthesia for mice. Key words : tribromoethanol;anesthetic;mice;effect |
购买人 | 会员级别 | 数量 | 属性 | 购买时间 |
---|---|---|---|---|
981****1791 | 普通会员 | 1 | 三溴乙醇(阿佛丁)(30ml) | 2023-05-31 10:14:19 |
962****7462 | 普通会员 | 1 | 规格型号(30ml/瓶(液体)) | 2021-08-05 16:58:12 |
997****3339 | 普通会员 | 1 | 规格型号(10ml*2/盒) | 2021-06-21 17:56:10 |
136****1711 | 普通会员 | 1 | 2021-05-26 09:40:33 |
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